Structural changes in the gut virome of patients with atherosclerotic cardiovascular disease

作者：Youshan Li, Jie Ma, Jinxin Meng, Shenghui Li, Yan Zhang, Wei You, Xulin Sai, Jianfeng Yang, Shuo Zhang, Wen Sun · 发表于：Microbiology Spectrum · 年份：2023 · DOI：10.1128/spectrum.01050-23 · 被引用次数：23 · 研究领域：Gut microbiota and health、Bacteriophages and microbial interactions、Respiratory viral infections research

ABSTRACT The gut microbiota is an important risk factor and therapeutic target in atherosclerotic cardiovascular disease (ACVD), a leading cause of morbidity and mortality worldwide. However, alterations in the gut viral community and its contribution to ACVD have rarely been investigated. In this study, we characterized and compared the gut viromes from the fecal metagenomes of 214 patients with ACVD and 171 healthy individuals using a reference-dependent virome approach. We revealed that ACVD patients exhibited a significant increase in viral richness at the family level and a visible alteration in overall virome structure regardless of host sex, age, or body mass index. At the viral operational taxonomic unit (vOTU) level, we identified 105 vOTUs that significantly increased in abundance in ACVD patients and 60 vOTUs that increased in abundance in healthy controls. A majority (43.8%) of the ACVD-enriched vOTUs were predicted to infect Streptococcaceae , Lachnospiraceae , Ruminococcaceae , and Enterobacteriaceae , and Streptococcaceae had tight correlations with the corresponding gut bacterial species, whereas a considerable proportion (35.0%) of the control-enriched vOTUs were Bacteroidaceae , Burkholderiaceae , and Lachnospiraceae phages. Functional analyses revealed five viral auxiliary metabolic genes that differed in frequency between ACVD-enriched and control-enriched vOTUs. Moreover, we identified gut viral signatures for ACVD discrimination and achieved an optimal a...