Evaluation of minimum-to-severe global and macrovesicular steatosis in human liver specimens: a portable ambient light-compatible spectroscopic probe

作者：Hao Guo, Ashley Stueck, Jason B. Doppenberg, Yun Suk Chae, Alexey B. Tikhomirov, Haishan Zeng, Marten A. Engelse, Boris Gala-López, Anita Mahadevan‐Jansen, Ian P.J. Alwayn, Andrea K. Locke, K. C. Hewitt · 发表于：medRxiv · 年份：2023 · DOI：10.1101/2023.12.04.23299259 · 被引用次数：4 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Liver Disease Diagnosis and Treatment、Organ Transplantation Techniques and Outcomes

Background & Aims Hepatic steatosis (HS), particularly macrovesicular steatosis (MaS), influences transplant outcomes. Accurate assessment of MaS is crucial for graft selection. While traditional assessment methods have limitations, non-invasive spectroscopic techniques like Raman and reflectance spectroscopy offer promise. This study aimed to evaluate the efficacy of a portable ambient light-compatible spectroscopic system in assessing global HS and MaS in human liver specimens. Methods A two-stage approach was employed on thawed snap-frozen human liver specimens under ambient room light: biochemical validation involving a comparison of fat content from Raman and reflectance intensities with triglyceride (TG) quantifications and histopathological validation, contrasting Raman-derived fat content with evaluations by an expert pathologist and an artificial intelligence (AI) algorithm. Raman and reflectance intensities were combined to discern significant (≥10%) discrepancies in global HS and MaS. Results The initial set of 16 specimens showed a positive correlation between Raman and reflectance-derived fat content and TG quantifications. The Raman system effectively differentiated minimum-to-severe global and macrovesicular steatosis in the subsequent 66 specimens. A dual-variable prediction algorithm, was developed, effectively classifying significant discrepancies (>10%) between AI-estimated global HS and pathologist-estimated MaS. Conclusion Our study established the...