Hydrogen-Rich Saline Regulates Microglial Phagocytosis and Restores Behavioral Deficits Following Hypoxia-Ischemia Injury in Neonatal Mice via the Akt Pathway

作者：Ke H, Liu D, Tao Li, Chu X, Xin D, Han Mao, Sheng Wang, Zhidong Wang · 发表于：DOAJ (DOAJ: Directory of Open Access Journals) · 年份：2020 · 被引用次数：2 · 研究领域：Hydrogen's biological and therapeutic effects、Anesthesia and Neurotoxicity Research、MicroRNA in disease regulation

Hongfei Ke1 ,* Dexiang Liu2 ,* Tingting Li,1 Xili Chu,1 Danqing Xin,1 Min Han,1 Shuanglian Wang,1 Zhen Wang1 1Department of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People’s Republic of China; 2Department of Medical Psychology and Ethics, School of Basic Medicine Sciences, Cheeloo College of Medicine, Shandong University, Jinan 250012, Shandong, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhen WangDepartment of Physiology, School of Basic Medical Sciences, Cheeloo College of Medicine, Shandong University, Jinan, Shandong 250012, People’s Republic of ChinaEmail wangzhen@sdu.edu.cnIntroduction: We have reported previously that hydrogen-rich saline (HS) plays a neuroprotective role in hypoxia-ischemia (HI) brain damage in newborn mice. However, the mechanisms for this neuroprotection resulting from HS remain unknown. In this study, we examined the potential for HS to exert effects upon microglial phagocytosis via involvement of the Akt signaling pathway as one of the neuroprotective mechanisms in response to neonatal HI.Methods: The HI brain injury model was performed on postnatal day (PND) 7 (modified Vannucci model). The acute brain damage was detected at 3 days after HI exposure. The behavioral and functional screening of the pups at PND11 and PND13 and their long-term outcomes (PND35, 28-days post-HI) were evaluated sensorimotor perfor...