Overexpression of an ART1-Interacting Gene OsNAC016 Improves Al Tolerance in Rice

作者：Fuhang Liu, Dan Ma, Jinyu Yu, Ran Meng, Zhigang Wang, Baolei Zhang, Xingxiang Chen, Lin Zhang, Liyun Peng, Jixing Xia · 发表于：International Journal of Molecular Sciences · 年份：2023 · DOI：10.3390/ijms242317036 · 被引用次数：11 · 研究领域：Aluminum toxicity and tolerance in plants and animals、Plant Stress Responses and Tolerance、Silicon Effects in Agriculture

Rice (Oryza sativa) exhibits tremendous aluminum (Al)-tolerance. The C2H2-transcription factor (TF) ART1 critically regulates rice Al tolerance via modulation of specific gene expression. However, little is known about the posttranscriptional ART1 regulation. Here, we identified an ART1-interacted gene OsNAC016 via a yeast two-hybrid (Y2H) assay. OsNAC016 was primarily expressed in roots and weakly induced by Al. Immunostaining showed that OsNAC016 was a nuclear protein and localized in all root cells. Knockout of OsNAC016 did not alter Al sensitivity. Overexpression of OsNAC016 resulted in less Al aggregation within roots and enhanced Al tolerance in rice. Based on transcriptomic and qRT-PCR evaluations, certain cell-wall-related or ART-regulated gene expressions such as OsMYB30 and OsFRDL4 were altered in OsNAC016-overexpressing plants. These results indicated that OsNAC016 interacts with ART1 to cooperatively regulate some Al-tolerance genes and is a critical regulatory factor in rice Al tolerance.