Maternal folic acid and vitamin B 12 supplementation during medium to late gestation promotes fetal development via improving placental antioxidant capacity, angiogenesis and amino acid transport

作者：Rui Zhou, Zhe Li, Fangyuan Chen, Tianle Gao, Xiaoling Zhang, Lingjie Huang, Yong Zhuo, Shengyu Xu, Yan Lin, Bin Feng, Lianqiang Che, De Wu, Zhengfeng Fang · 发表于：Journal of the Science of Food and Agriculture · 年份：2023 · DOI：10.1002/jsfa.13171 · 被引用次数：17 · 研究领域：Folate and B Vitamins Research、Pregnancy and preeclampsia studies、Gestational Diabetes Research and Management

Abstract BACKGROUND Folic acid and vitamin B 12 (FV), being B vitamins, not only facilitate the remethylation of homocysteine (Hcy) but also contribute to embryonic development. This study aimed to assess the impact of FV supplementation during late pregnancy on sows' reproductive performance, amino acid metabolism, placental angiogenesis, and related parameters. Twenty primiparous sows at day 60 of gestation were randomly allocated to two groups: a basal diet (CON) group and a group receiving a basal diet supplemented with folic acid at 20 ppm and vitamin B 12 at 125 ppb. RESULTS The findings revealed that dietary FV supplementation significantly reduced the incidence of intrauterine growth retardation compared to the CON group ( P < 0.05). Furthermore, it led to a decrease in the Hcy levels in umbilical cord serum ( P < 0.05) and activation of the placental mechanistic target of rapamycin complex 1 (mTORC1) signaling pathway ( P < 0.05). Additionally, FV supplementation lowered placental malondialdehyde levels ( P < 0.05) and increased the expression of placental thioredoxin ( P = 0.05). Moreover, maternal FV supplementation notably elevated placental vascular density ( P < 0.05) and the expression of sodium‐coupled neutral amino acid transporter 2 (SNAT2) ( P < 0.05), as well as amino acid concentrations in umbilical cord blood ( P < 0.05). CONCLUSION Maternal FV supplementation during medium to late gestation reduced Hcy levels in umbilical cord blood...