Retinoic Acid–PPARα Mediates β-Carotene Resistance to Placental Dysfunction Induced by Deoxynivalenol

作者：Longmiao Zhang, Shuangbo Huang, Kaidi Ma, Yi‐Ling Chen, Tanghong Wei, Hongxuan Ye, Junyi Wu, Liudan Liu, Jinping Deng, Hefeng Luo, Chengquan Tan · 发表于：Journal of Agricultural and Food Chemistry · 年份：2023 · DOI：10.1021/acs.jafc.3c06647 · 被引用次数：5 · 研究领域：Retinoids in leukemia and cellular processes、Antioxidant Activity and Oxidative Stress、Fatty Acid Research and Health

Deoxynivalenol (DON), one of the most polluted mycotoxins in the environment and food, has been proven to have strong embryonic and reproductive toxicities. However, the effects of DON on placental impairment and effective interventions are still unclear. This study investigated the effect of β-carotene on placental functional impairment and its underlying molecular mechanism under DON exposure. Adverse pregnancy outcomes were caused by intraperitoneal injection of DON from 13.5 to 15.5 days of gestation in mice, resulting in higher enrichment of DON in placenta than in other tissue samples. Interestingly, 0.1% β-carotene dietary supplementation could significantly alleviate DON-induced pregnancy outcomes. Additionally, in vivo and in vitro placental barrier models demonstrated the association of DON-induced placental function impairment with placental permeability barrier disruption, angiogenesis impairment, and oxidative stress induction. Moreover, β-carotene regulated DON-induced placental toxicity by activating the expressions of claudin 1, zonula occludens -1, and vascular endothelial growth factor-A through retinoic acid–peroxisome proliferator-activated receptor α signaling.