2D Hierarchical Microbarcodes with Expanded Storage Capacity for Optical Multiplex and Information Encryption

作者：Yujie Xie, Zaizai Tong, Tianlai Xia, Joshua C. Worch, Julia Y. Rho, Andrew P. Dove, Rachel K. O’Reilly · 发表于：Advanced Materials · 年份：2023 · DOI：10.1002/adma.202308154 · 被引用次数：58 · 研究领域：Luminescence and Fluorescent Materials、Advanced biosensing and bioanalysis techniques、Nanoplatforms for cancer theranostics

The design of nanosegregated fluorescent tags/barcodes by geometrical patterning with precise dimensions and hierarchies could integrate multilevel optical information within one carrier and enhance microsized barcoding techniques for ultrahigh-density optical data storage and encryption. However, precise control of the spatial distribution in micro/nanosized matrices intrinsically limits the accessible barcoding applications in terms of material design and construction. Here, crystallization forces are leveraged to enable a rapid, programmable molecular packing and rapid epitaxial growth of fluorescent units in 2D via crystallization-driven self-assembly. The fluorescence encoding density, scalability, information storage capacity, and decoding techniques of the robust 2D polymeric barcoding platform are explored systematically. These results provide both a theoretical and an experimental foundation for expanding the fluorescence storage capacity, which is a longstanding challenge in state-of-the-art microbarcoding techniques and establish a generalized and adaptable coding platform for high-throughput analysis and optical multiplexing.