Physiological, Epigenetic, and Transcriptome Analyses Provide Insights into the Responses of Wheat Seedling Leaves to Different Water Depths under Flooding Conditions

作者：Bo Li, Wei Hua, Shuo Zhang, Le Xu, Caixian Yang, Zhanwang Zhu, Ying Guo, Meixue Zhou, Chunhai Jiao, Yanhao Xu · 发表于：International Journal of Molecular Sciences · 年份：2023 · DOI：10.3390/ijms242316785 · 被引用次数：24 · 研究领域：Plant responses to water stress、Plant Stress Responses and Tolerance、Rice Cultivation and Yield Improvement

Flooding stress, including waterlogging and submergence, is one of the major abiotic stresses that seriously affects the growth and development of plants. In the present study, physiological, epigenetic, and transcriptomic analyses were performed in wheat seedling leaves under waterlogging (WL), half submergence (HS), and full submergence (FS) treatments. The results demonstrate that FS increased the leaves’ hydrogen peroxide (H2O2) and malondialdehyde (MDA) contents and reduced their chlorophyll contents (SPAD), photosynthetic efficiency (Fv/Fm), and shoot dry weight more than HS and WL. In addition, FS increased catalase (CAT) and peroxidase (POD) activities more than HS and WL. However, there were no significant differences in the contents of H2O2, MDA, SPAD, and Fv/Fm, and the activities of superoxide dismutase (SOD) and POD between the HS and WL treatments. The changes in DNA methylation were related to stress types, increasing under the WL and HS treatments and decreasing under the FS treatment. Additionally, a total of 9996, 10,619, and 24,949 genes were differentially expressed under the WL, HS, and FS treatments, respectively, among which the ‘photosynthesis’, ‘phenylpropanoid biosynthesis’, and ‘plant hormone signal transduction’ pathways were extensively enriched under the three flooding treatments. The genes involved in these pathways showed flooding-type-specific expression. Moreover, flooding-type-specific responses were observed in the three conditions, includi...