A papain-like cysteine protease-released small signal peptide confers wheat resistance to wheat yellow mosaic virus

作者：Peng Liu, Chaonan Shi, Shuang Liu, Jiajia Lei, Qisen Lu, Haichao Hu, Yan Ren, Ning Zhang, Congwei Sun, Lu Chen, Yaoyao Jiang, Lixiao Feng, Tianye Zhang, Kaili Zhong, Jiaqian Liu, Juan Zhang, Zhuo Zhang, Bingjian Sun, Jianping Chen, Yimiao Tang, Feng Chen, Jian Yang · 发表于：Nature Communications · 年份：2023 · DOI：10.1038/s41467-023-43643-y · 被引用次数：41 · 研究领域：Plant Virus Research Studies、Insect Resistance and Genetics、Plant-Microbe Interactions and Immunity

Wheat yellow mosaic virus (WYMV), a soil-borne pathogen, poses a serious threat to global wheat production. Here, we identify a WYMV resistance gene, TaRD21A, that belongs to the papain-like cysteine protease family. Through genetic manipulation of TaRD21A expression, we establish its positive role in the regulation of wheat to WYMV resistance. Furthermore, our investigation shows that the TaRD21A-mediated plant antiviral response relies on the release of a small peptide catalyzed by TaRD21A protease activity. To counteract wheat resistance, WYMV-encoded nuclear inclusion protease-a (NIa) suppress TaRD21A activity to promote virus infection. In resistant cultivars, a natural variant of TaRD21A features a glycine-to-threonine substitution and this substitution enables the phosphorylation of threonine, thereby weakening the interaction between NIa and TaRD21A, reinforcing wheat resistance against WYMV. Our study not only unveils a WYMV resistance gene but also offers insights into the intricate mechanisms underpinning resistance against WYMV.