570. Impact of a Blood Culture Direct Identification Procedure on Time to Identification for Gram-Negative Organisms and Antimicrobial Management in Bloodstream Infections

作者：Corwin Coppinger, Ashley L Cubillos · 发表于：Open Forum Infectious Diseases · 年份：2023 · DOI：10.1093/ofid/ofad500.639 · 被引用次数：1 · 研究领域：Bacterial Identification and Susceptibility Testing、Clinical Laboratory Practices and Quality Control、Infective Endocarditis Diagnosis and Management

Abstract Background Multiplex polymerase chain reaction (PCR) and microarray diagnostic methods for rapid identification (ID) in Gram-negative bloodstream infections (GNBSI) remain unavailable in many healthcare facilities. Use of other methods such as matrix-associated laser desorption/ionization time-of-flight (MALDI-TOF) along with rapid sample preparation from positive blood cultures, may decrease time to ID and targeted antimicrobial therapy in BSI. This study compares time to ID and final susceptibilities of Gram-negative bacteria (GNB) in positive blood cultures using a rapid MALDI-TOF direct ID process versus conventional culture methods, as well as impact on antimicrobial therapy in GNBSI. Methods This retrospective cohort included patients ≥18 years of age who had a positive blood culture with a GNB from January 1 through December 31, 2022, at a hospital serviced by the microbiology lab at Banner University Medical Center Phoenix (conventional ID) or Banner University Medical Center Tucson (direct ID). Direct ID consisted of using MALDI-TOF for immediate ID of a centrifuged sample from a GNB-positive blood culture bottle. The primary outcome was time to GNB ID between the two groups. Secondary outcomes included time from positive blood culture to susceptibility reporting, definitive antibiotic regimen, and hospital discharge. Statistical analysis performed with Wilcoxon Rank-Sum testing for continuous data, and Chi square testing for nominal data. Results A total of...