Genome editing of a dominant resistance gene for broad‐spectrum resistance to bacterial diseases in rice without growth penalty

作者：Meixia Wang, Shaofang Li, Huayang Li, Congfeng Song, Wenya Xie, Shimin Zuo, Xueping Zhou, Changyong Zhou, Zhiyuan Ji, Huanbin Zhou · 发表于：Plant Biotechnology Journal · 年份：2023 · DOI：10.1111/pbi.14233 · 被引用次数：32 · 研究领域：Plant Pathogenic Bacteria Studies、Plant-Microbe Interactions and Immunity、Legume Nitrogen Fixing Symbiosis

Bacterial blight (BB) and bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. oryzae (Xoo) and Xanthomonas oryzae pv. oryzicola (Xoc), respectively, are the two most devastating bacterial diseases of rice worldwide. Both bacterial pathogens infect rice plants relying on type III secreted transcriptional activation-like effectors (TALEs) that bind to specific effector binding elements (EBEs) in the promoter of susceptibility (S) genes, and activate its expressions for disease development (Chen et al., 2010). To counteract BB, rice has evolved a unique type of executor resistance (R) genes (Xa7, Xa10, Xa23, etc.), which can specifically trap certain Xoo TALEs via EBEs within their promoters and trigger strong hypersensitive response (HR). Previously, we verified that gene correction of xa23 with EBEAvrXa23 restored its function in triggering defence responses against Xoo invasion (Wei et al., 2021). Considering Xa23-mediated resistance to BB has been overcome by new Xoo isolates in the field in recent years, and no natural R genes against Xoc have been identified in rice; here, we investigated whether an EBE-stacking-in-the-promoter strategy could be employed in the molecular rice breeding for durable and broad-spectrum resistance to both BB and BLS by genome-editing technology. Sequencing analysis of the xa23 locus in the commercial rice cultivar Nangeng 46 (N46) revealed that it shared an identical coding region with Xa23 in CBB23, whereas it lacked the complete EBE...