Natural allelic diversities of GmPrx16 confer drought tolerance in soybean

作者：Zhifang Zhang, Junkui Ma, Xia Yang, Zhi Liu, Yucheng Liu, Xueyi Liu, Shan Liang, Zongbiao Duan, Zheng Wang, Xiaoyue Yang, Yan Long, Min Zhang, Shulin Liu, Zhixi Tian · 发表于：Plant Biotechnology Journal · 年份：2023 · DOI：10.1111/pbi.14249 · 被引用次数：31 · 研究领域：Soybean genetics and cultivation、Plant pathogens and resistance mechanisms、Legume Nitrogen Fixing Symbiosis

Drought has a serious impact on agricultural production, which can result in production losses ranging between 30% and 90%, depending on the crop species (Hussain et al., 2019; Lesk et al., 2016). Soybean [Glycine max (L.) Merr.] is an important crop that provides primary vegetable oil and protein as well as a supplement in livestock feed worldwide (Wilson, 2008). However, soybean is very sensitive to drought (Dong et al., 2019), and it has been revealed that from 1983 to 2009 approximately 67 Mha of harvested soybean areas experienced drought stress, resulting in a 7% total yield loss (Kim et al., 2019). To identify the genes and corresponding beneficial alleles conferring drought tolerance in soybean germplasms, we performed genome-wide association study (GWAS) using a panel of 585 soybean accessions, which had been genotyped in our previous work (Fang et al., 2017). The drought tolerance values of these 585 accessions were evaluated in the field in Shanxi Province, China (Figure S1, Table S1). GWAS identified one significant association locus on chromosome 16 ranging from 32 206 964 bp to 32 458 483 bp that covered 23 genes (Figure 1a, Table S2). Haplotype investigation showed that a C to G nonsynonymous SNP in Glyma.16G164400 (encoding a peroxidase, named as GmPrx16) was highly associated with the drought tolerance variation in soybean germplasm, suggesting that GmPrx16 might be the candidate gene in the association locus on chromosome 16 (Figure 1b). GmPrx16 was highly e...