Performance of CHROMagar ESBL media for the surveillance of extended-spectrum cephalosporin-resistant Enterobacterales (ESCrE) from rectal swabs in Botswana

作者：Naledi Mannathoko, Ebbing Lautenbach, Mosepele Mosepele, Dimpho Otukile, Kgotlaetsile Sewawa, Laurel Glaser, Leigh Cressman, Laura Cowden, Kevin Alby, Anne Jaskowiak‐Barr, Robert Gross, Margaret Mokomane, Giacomo Maria Paganotti, Ashley Styczynski, Rachel M. Smith, Evan S. Snitkin, Tiffany Wan, Warren B. Bilker, Melissa Richard‐Greenblatt · 发表于：Journal of Medical Microbiology · 年份：2023 · DOI：10.1099/jmm.0.001770 · 被引用次数：11 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial Identification and Susceptibility Testing、Antibiotic Use and Resistance

Introduction. Lack of laboratory capacity hampers consistent national antimicrobial resistance (AMR) surveillance. Chromogenic media may provide a practical screening tool for detection of individuals colonized by extended-spectrum beta-lactamase (ESBL)-producing organisms. Hypothesis. CHROMagar ESBL media represent an adequate screening method for the detection of extended-spectrum cephalosporin-resistant Enterobacterales (ESCrE), isolated from rectal swabs. Aim. To evaluate the performance of CHROMagar ESBL media to accurately identify ESCrE isolates from rectal swab samples attained from hospitalized and community participants. Methodology. All participants provided informed consent prior to enrolment. Rectal swabs from 2469 hospital and community participants were inoculated onto CHROMagar ESBL. The performance of CHROMagar ESBL to differentiate Escherichia coli and Klebsiella spp., Enterobacter spp. and Citrobacter spp. (KEC spp.) as well as select for extended-spectrum cephalosporin resistance were compared to matrix-assisted laser desorption/ionization-time-of-flight MS (MALDI-TOF-MS) and VITEK-2 automated susceptibility testing. Results. CHROMagar ESBL had a positive and negative agreement of 91.2 % (95 % CI, 88.4–93.3) and 86.8 % (95 % CI, 82.0–90.7) for E. coli and 88.1 % (95 % CI 83.2–92.1) and 87.6 % (95 % CI 84.7–90.2) for KEC spp. differentiation, respectively, when compared to species ID by MALDI-TOF-MS. When evaluated for phenotypic susceptibilities (VITEK-2),...