A laser-induced graphene-based electrochemical immunosensor for nucleic acid methylation detection

作者：Jingyi Guo, Mei Zhao, Chen Chen, Fang Wang, Zilin Chen · 发表于：The Analyst · 年份：2023 · DOI：10.1039/d3an01628e · 被引用次数：29 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA modifications and cancer、Nanopore and Nanochannel Transport Studies

= 0.9903) for 5mC-ssDNA. Our method has good selectivity toward different detection targets of nucleic acid chains, stability for long-term storage and consecutive scanning (RSD of 9.42% and 2.08%, respectively) and reproducibility of 5 electrodes (RSD of 6.85%). This method utilizes gold-sulfur bonding to immobilize the detection target, which improves the conductivity of the LIG electrode and introduces an amplified portion of the signal by taking advantage of antigen-antibody specific binding. Thus, dual detection of m6A-RNA and 5mC-ssDNA was realized. Importantly, this approach is successfully applied for the detection of targets in spiked samples extracted from HeLa cells, suggesting its potential for clinical applications and providing a new perspective for the development of point-of care testing (POCT) techniques.