The influence of inflammation on the characteristics of adipose-derived mesenchymal stem cells (ADMSCs) and tissue repair capability in a hepatic injury mouse model

作者：Jingfang Xiao, Xiaoyuan Gong, Zhenlan Fu, Xiongbo Song, Qinghua Ma, Jingya Miao, Ruili Cai, Zexuan Yan, Shuai Wang, Qian Li, Yaokai Chen, Yaokai Chen, Liu Yang, Xiu‐Wu Bian, Yemiao Chen, Yemiao Chen · 发表于：Stem Cell Research & Therapy · 年份：2023 · DOI：10.1186/s13287-023-03532-z · 被引用次数：13 · 研究领域：Mesenchymal stem cell research、Osteoarthritis Treatment and Mechanisms、Bone and Joint Diseases

BACKGROUND: Mesenchymal stem cells (MSCs) are adult stem cells with self-renewal and multi-directional differentiation potential and possess the functions of immunomodulation, regulation of cell growth, and repair of damage. Over recent years, MSCs have been found to regulate the secretion of inflammatory factors and to exert regulatory effects on various lymphocytes in inflammatory states, and on the subsequent repair of tissue damage caused by inflammation. In the present study, we analyzed the effects of tissue inflammation on the characteristics of MSCs. METHODS: Human fat derived from the infrapatellar fat pad (IPFP) of knees with differing degrees of inflammation was extracted from specimens derived from total knee arthroplasties. HE and immunohistochemical staining was performed to directly observe the evidence and degree of inflammation in human infrapatellar fat pad tissue in order to classify MSCs cells, by their origin, into highly inflamed and lowly inflamed groups, and to study the effect of tissue inflammation on cell acquisition rates via cellular counting data. Flow cytometry assays were performed to investigate the effect of tissue inflammation on MSC surface marker expression. Trilineage differentiation, including osteogenesis, adipogenesis, and chondrogenesis, was performed to assess the effect of tissue inflammation on the ability of MSCs to undergo directed differentiation. The effect of tissue inflammation on the ability of MSCs to proliferate was invest...