Time-Course Transcriptome Analysis of Aquilegia vulgaris Root Reveals the Cell Wall’s Roles in Salinity Tolerance

作者：Yun Bai, Haihang Yu, Lifei Chen, Yuan Meng, Yanmei Ma, Di Wang, Ying Qian, Dongyang Zhang, Xiaoyu Feng, Yunwei Zhou · 发表于：International Journal of Molecular Sciences · 年份：2023 · DOI：10.3390/ijms242216450 · 被引用次数：13 · 研究领域：Plant Stress Responses and Tolerance、Plant Molecular Biology Research、Plant nutrient uptake and metabolism

Salt stress has a considerable impact on the development and growth of plants. The soil is currently affected by salinisation, a problem that is becoming worse every year. This means that a significant amount of salt-tolerant plant material needs to be added. Aquilegia vulgaris has aesthetically pleasing leaves, unique flowers, and a remarkable tolerance to salt. In this study, RNA-seq technology was used to sequence and analyse the transcriptome of the root of Aquilegia vulgaris seedlings subjected to 200 mM NaCl treatment for 12, 24, and 48 h. In total, 12 Aquilegia vulgaris seedling root transcriptome libraries were constructed. At the three time points of salt treatment compared with the control, 3888, 1907, and 1479 differentially expressed genes (DEGs) were identified, respectively. Various families of transcription factors (TFs), mainly AP2, MYB, and bHLH, were identified and might be linked to salt tolerance. Gene Ontology (GO) analysis of DEGs revealed that the structure and composition of the cell wall and cytoskeleton may be crucial in the response to salt stress. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of the DEGs showed a significant enrichment of the pentose and glucuronate interconversion pathway, which is associated with cell wall metabolism after 24 and 48 h of salt treatment. Based on GO and KEGG analyses of DEGs, the pentose and glucuronate interconversion pathway was selected for further investigation. AP2, MYB, and bHLH were found to be co...