Individual Human Hair Keratin Rapidly Extracted Through the Advanced Oxidation Process and Their Hemostatic and Wound Healing Evaluations

作者：Jia Deng, Xiaojing Yuan, Lian Wang, Yanqi Sun, Hao He, Zhenyi Wu, Zibin Gao, Jingou Ji, Bochu Wang, Shilei Hao · 发表于：ACS Applied Polymer Materials · 年份：2023 · DOI：10.1021/acsapm.3c02587 · 被引用次数：2 · 研究领域：Dyeing and Modifying Textile Fibers、Skin Protection and Aging、Dermatologic Treatments and Research

Autogenous keratin is a promising material for personalized wound healing applications. However, the common keratin extraction methods are time-consuming, and the variations in performance between different individual keratins are not clear. Herein, we developed TiO 2 photocatalytic-assisted H 2 O 2 oxidation strategy (H 2 O 2 /TiO 2 /UV), an advanced oxidation method, to rapidly extract human hair keratins within 2 h. The H 2 O 2 /TiO 2 /UV method for keratin extraction showed a higher protein yield compared to the H 2 O 2 method. Keratins obtained from the developed method exhibited typical chemical and secondary structures. Furthermore, individual keratins from four young girls were extracted by using the H 2 O 2 /TiO 2 /UV method, and individual keratin/PCL films were successfully fabricated within 5 h. More importantly, individual keratin films displayed superior hemostatic performance and wound healing in vivo compared to that of keratin extracted via the H 2 O 2 method. Interestingly, the four individual keratin films exhibited similar hemostatic efficiency but distinct wound-healing properties in terms of epidermal thickness and density of skin appendages. These data indicated that the developed H 2 O 2 /TiO 2 /UV method is an efficient and feasible approach to extract human hair keratins, which provides an intriguing possibility to explore personalized materials for promoting wound healing.