3-D culture of human lung fibroblasts decreases proliferative and increases extracellular matrix remodeling genes

作者：Nataliya Migulina, Roderick H.J. de Hilster, Sabine Bartel, Rolf Vedder, Maarten van den Berge, Anika Nagelkerke, Wim Timens, Martin C. Harmsen, Machteld N. Hylkema, Corry‐Anke Brandsma, Janette K. Burgess · 发表于：American Journal of Physiology-Cell Physiology · 年份：2023 · DOI：10.1152/ajpcell.00374.2023 · 被引用次数：9 · 研究领域：Hippo pathway signaling and YAP/TAZ、Cellular Mechanics and Interactions、Cancer Cells and Metastasis

With the introduction of complex three-dimensional (3-D) lung models, comes a need for understanding cellular behavior in these models. We compared gene expression profiles of human lung fibroblasts grown on two-dimensional (2-D) collagen I-coated surfaces with those in 3-D collagen I hydrogels. RNA sequencing and subsequent pathway analyses showed decreased proliferation, increased extracellular matrix (ECM) remodeling, and altered Hippo signaling and ECM deposition-related gene signatures. These findings highlight unique responses of fibroblasts in 3-D models.