Moutai Distiller's grains Polyphenol extracts and rutin alleviate DSS-induced colitis in mice: Modulation of gut microbiota and intestinal barrier function （R2）

作者：Jinhu Chen, Caili Zhao, Yong-su Li, Yubo Yang, Jian‐Guang Luo, Chao Zhang, Li Wang · 发表于：Heliyon · 年份：2023 · DOI：10.1016/j.heliyon.2023.e22186 · 被引用次数：27 · 研究领域：Gut microbiota and health、Probiotics and Fermented Foods、Neurological Disease Mechanisms and Treatments

Distiller's grains, byproducts of the brewing process, represent a valuable resource for extracting natural phenolic compounds due to their significant global production. This study presents the first evidence of the protective effects of Moutai distiller's grain polyphenol extract (MDGP) on dextran sulfate sodium (DSS)-induced colitis in mice. These protective effects manifest predominantly through the amelioration of general colitis indices and histopathological improvements. Utilizing liquid chromatography-high-resolution electrospray ionization mass spectrometry (LC-HR-ESI-MS), the main components of MDGP were identified as rutin, quercetin, naringenin, and dihydroquercetin. Moreover, a novel mechanism was elucidated by which rutin, the primary active component of MDGP, alleviates DSS-induced colitis. Assessment of intestinal barrier function, microbial sequencing, fecal transplantation, and antibiotic depletion experiments revealed that rutin suppresses the abundance of pathogenic bacteria ( Helicobacter , Klebsiella , and Veillonella ) while promoting the proliferation of beneficial bacteria ( Ruminococcus_torques_group , Lachnoclostridium , and norank_f__Muribaculaceae ). This modulation culminates in elevated butyric acid concentrations within short-chain fatty acids (SCFAs), amplified integrity of tight (ZO-1, occludin) and adherent (E-cadherin, β-catenin) junctional complexes, fortified intestinal barrier function, and diminished intestinal inflammation.This investi...