Circadian gene Per3 promotes astroblastoma progression through the P53/BCL2/BAX signalling pathway

作者：Qingqing Wang, Huaifeng Liu, Zhiheng Wang, Yuxin Chen, Shujing Zhou, Xinyi Hu, Yangfei Xu, Xinxin Zhang, Yuanyuan Wang, Yu Gao, Shujing Li · 发表于：Gene · 年份：2023 · DOI：10.1016/j.gene.2023.147978 · 被引用次数：12 · 研究领域：Circadian rhythm and melatonin、Genetics, Aging, and Longevity in Model Organisms、Spaceflight effects on biology

The key circadian genes, Period1(Per1), Period2(Per2), and Period3(Per3), constitute the mammalian Period gene family. The abnormal expression of Per1 and Per2 is closely related to tumor development, but there are few reports on Per3 and tumorigenesis. This study was conducted to determine whether the abnormal expression of Per3 could influence the progression of astroblastoma. The results indicated that the expression level of Per3 was increased in astroblastoma cells, and the high expression of Per3 was correlated with the poor overall survival time of glioma patients. The role of Per3 in astroblastoma cells was then investigated using two approaches: interference and overexpression. The interference of Per3 inhibited astroblastoma cell proliferation by inducing the cell cycle at the S phase. The interference of Per3 inhibited the migration and invasion of astroblastoma cells, while promoted the astroblastoma cell apoptosis and the expression of the apoptosis genes Cleaved-CASP3, P53, and BAX. The overexpression of Per3 promoted proliferation by affecting the S phase distribution of the astroblastoma cell cycle. The overexpression of Per3 promoted the migration and invasion of astroblastoma cells, while inhibited the astroblastoma cell apoptosis and the expression of apoptosis genes Cleaved-CASP3, P53, and BAX. RNA-seq analysis showed that the interference of Per3 in astrocytoma cells resulted in significant changes in the expression levels of 764 genes. Among the differen...