Recombinase Polymerase Amplification-Based Biosensors for Rapid Zoonoses Screening

作者：Xingzhu Feng, Yan Liu, Yang Zhao, Zhe Sun, Ning Xu, Chen Zhao, Wei Xia · 发表于：International Journal of Nanomedicine · 年份：2023 · DOI：10.2147/ijn.s434197 · 被引用次数：42 · 研究领域：Biosensors and Analytical Detection、Advanced biosensing and bioanalysis techniques、Bacteriophages and microbial interactions

Recent, outbreaks of new emergency zoonotic diseases have prompted an urgent need to develop fast, accurate, and portable screening assays for pathogen infections. Recombinase polymerase amplification (RPA) is sensitive and specific and can be conducted at a constant low temperature with a short response time, making it especially suitable for on-site screening and making it a powerful tool for preventing or controlling the spread of zoonoses. This review summarizes the design principles of RPA-based biosensors as well as various signal output or readout technologies involved in fluorescence detection, lateral flow assays, enzymatic catalytic reactions, spectroscopic techniques, electrochemical techniques, chemiluminescence, nanopore sequencing technologies, microfluidic digital RPA, and clustered regularly interspaced short palindromic repeats/CRISPR-associated systems. The current status and prospects of the application of RPA-based biosensors in zoonoses screening are highlighted. RPA-based biosensors demonstrate the advantages of rapid response, easy-to-read result output, and easy implementation for on-site detection, enabling development toward greater portability, automation, and miniaturization. Although there are still problems such as high cost with unstable signal output, RPA-based biosensors are increasingly becoming one of the most important means of on-site pathogen screening in complex samples involving environmental, water, food, animal, and human samples for ...