Intensity Interrogation-Based High-Sensitivity Surface Plasmon Resonance Imaging Biosensor for Apoptosis Detection in Cancer

作者：Xin Yuan, Zhenxiao Niu, Lang Liu, Youjun Zeng, Lin Ma, Zhaogang Nie, Zhen Tian, Dongyun Kai, Fangteng Zhang, Guanyu Liu, Siwei Li, Zhengqiang Yuan · 发表于：Biosensors · 年份：2023 · DOI：10.3390/bios13100946 · 被引用次数：14 · 研究领域：Advanced biosensing and bioanalysis techniques、Plasmonic and Surface Plasmon Research、Advanced Biosensing Techniques and Applications

Intensity interrogation-based surface plasmon resonance imaging (ISPRi) sensing has a simple schematic design and is the most widely used surface plasmon resonance technology at present. In this study, we report the successful development of a novel high-sensitivity ISPRi biosensor and its application for apoptosis detection in cancer cells. By optimizing the excitation wavelength and excitation angle, we achieved a refractive index resolution (RIR) of 5.20 × 10−6 RIU. Importantly, the biosensor has been tested and validated for high-throughput and label-free detection of activated caspase-3 with its specific inhibitor Z-DEVD-FMK in apoptotic cells. Therefore, this study describes a novel molecular imaging system to monitor apoptosis in cancers for disease diagnosis and/or evaluation of therapeutic efficacy of anti-cancer drugs.