[A dry-reagent assay to rapidly detect Mycobacterium tuberculosis using loop-mediated isothermal amplification].

作者：B H Li, Ying Zhang, Sha Tao, Yanan Guo, Qian Liu, Quan Sun · 发表于：PubMed · 年份：2023 · DOI：10.3760/cma.j.cn112150-20230623-00488 · 研究领域：Respiratory viral infections research、Biosensors and Analytical Detection

, mumps virus, adenovirus (type 3), adenovirus (type 7), respiratory syncytial virus B and parainfluenza virus type 2, indicating a good specificity. Furthermore, a dry-reagent assay was developed using air-drying and freeze-drying process. The performance of dried reagents did not change after 10 days storage at 50 ℃, meaning the dried reagents could be stored at room temperature (25 ℃) for more than six months. The dry-reagent LAMP assay also successfully amplified MTB DNA from several clinical samples within 20 min. In conclusion, the developed LAMP assay together with isothermal amplifier could rapidly detection MTB.