Assembling of Gold Nanoparticles in Core–Shell Zeolite Imidazolate Framework Crystals for In Situ Electrochemical Detection of H 2 O 2 Released from Living Cells

作者：Zekun Wang, Liyan Yan, Ying Liu, Jun Wang, Dongxiang Li, Yiwei Wang, Xiaoyu Wang, Lin Li, Sagar Regmi, Jian Ju, Tianrong Zhan · 发表于：ACS Sustainable Chemistry & Engineering · 年份：2023 · DOI：10.1021/acssuschemeng.3c03663 · 被引用次数：15 · 研究领域：Electrochemical sensors and biosensors、Electrochemical Analysis and Applications、Advanced biosensing and bioanalysis techniques

The quantification of hydrogen peroxide (H 2 O 2 ), a vital biomarker in pathogenesis released from live cells, is of importance to life science. This work presents a method to assemble gold nanoparticles (AuNPs) in core–shell zeolite imidazolate framework (ZIF) crystals by the direct growth of ZIF-67 on ZIF-8 seeds (AuNPs/ZIF-8@ZIF-67). With a large surface, abundant catalytic sites, enhanced conductivity, and good dispersity of components, the AuNPs/ZIF-8@ZIF-67-modified glassy carbon electrode (AuNPs/ZIF-8@ZIF-67/GCE) reveals excellent catalytic activity for the electrochemical reduction of H 2 O 2 . The modified electrode exhibits a wide linear range of 0.05–120 μM and a low detection limit of 0.016 μM (S/N = 3) with excellent selectivity, stability, and reproducibility. The corresponding electrochemical sensor has been successfully used for the practical detection of H 2 O 2 released from living human embryonic kidney 293 (HEK293) cells and nonmetastatic breast cancer (MCF-7) cells under optimal conditions. This sensor demonstrates the potential for in situ detection applications in complicated biological systems.