Selective BET inhibitor RVX ‐208 ameliorates periodontal inflammation and bone loss

作者：Mingxu Sun, Nicholas P. Clayton, Sheikh Alam, Niels C. Asmussen, Andrew Wong, Jin Ha Kim, Gary Luong, Sasan Mokhtari, David Pellei, Caroline K. Carrico, Zvi Schwartz, Barbara D. Boyan, William V. Giannobile, Sinem E. Sahingur, Lin Zhao · 发表于：Journal Of Clinical Periodontology · 年份：2023 · DOI：10.1111/jcpe.13887 · 被引用次数：9 · 研究领域：Protein Degradation and Inhibitors、Oral and gingival health research、Chromatin Remodeling and Cancer

AIM: To determine the effects of RVX-208, a selective bromodomain and extra-terminal domain (BET) inhibitor targeting bromodomain 2 (BD2), on periodontal inflammation and bone loss. MATERIALS AND METHODS: Macrophage-like cells (RAW264.7) and human gingival epithelial cells were challenged by Porphyromonas gingivalis (Pg) with or without RVX-208. Inflammatory gene expression and cytokine production were measured by reverse transcription polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. RAW264.7 cells were induced to osteoclast differentiation. After RVX-208 treatment, osteoclast differentiation was evaluated by histology, tartrate-resistant-acid-phosphatase (TRAP) activity and the expression of osteoclast-specific genes. The effect of RVX-208 on osteoclast transcriptome was studied by RNA sequencing. Periodontitis was induced in rats by ligature and local RVX-208 treatment was administered every other day. Alveolar bone loss was measured by micro-computed tomography. RESULTS: RVX-208 inhibited inflammatory gene expression and cytokine production in Pg-infected cells. Osteoclast differentiation was inhibited by RVX-208, as evidenced by reduced osteoclast number, TRAP activity and osteoclast-specific gene expression. RVX-208 displayed a more selective and less profound suppressive impact on transcriptome compared with pan-BET inhibitor, JQ1. RVX-208 administration prevented the alveolar bone loss in vivo. CONCLUSIONS: RVX-208 regulated both upstream ...