Transcriptome analysis of Haemaphysalis flava female using Illumina HiSeq 4000 sequencing: de novo assembly, functional annotation and discovery of SSR markers

作者：Min Kyu Sang, Hongray Howrelia Patnaik, Jie Eun Park, Dae Kwon Song, Jun Yang Jeong, Chan Eui Hong, Young Tae Kim, Hyeon Jun Shin, Liu Ziwei, Hee Ju Hwang, So‐Young Park, Se Won Kang, Seung‐Hwan Park, Sung‐Jae Cha, Jung Ho Ko, E‐Hyun SHIN, Hong Seog Park, Yong Hun Jo, Yeon Soo Han, Bharat Bhusan Patnaik, Yong Seok Lee · 发表于：Parasites & Vectors · 年份：2023 · DOI：10.1186/s13071-023-05923-w · 被引用次数：9 · 研究领域：Viral Infections and Vectors、Vector-borne infectious diseases、Invertebrate Immune Response Mechanisms

BACKGROUND: Ticks are ectoparasites capable of directly damaging their hosts and transmitting vector-borne diseases. The ixodid tick Haemaphysalis flava has a broad distribution that extends from East to South Asia. This tick is a reservoir of severe fever with thrombocytopenia syndrome virus (SFTSV) that causes severe hemorrhagic disease, with cases reported from China, Japan and South Korea. Recently, the distribution of H. flava in South Korea was found to overlap with the occurrence of SFTSV. METHODS: This study was undertaken to discover the molecular resources of H. flava female ticks using the Illumina HiSeq 4000 system, the Trinity de novo sequence assembler and annotation against public databases. The locally curated Protostome database (PANM-DB) was used to screen the putative adaptation-related transcripts classified to gene families, such as angiotensin-converting enzyme, aquaporin, adenylate cyclase, AMP-activated protein kinase, glutamate receptors, heat shock proteins, molecular chaperones, insulin receptor, mitogen-activated protein kinase and solute carrier family proteins. Also, the repeats and simple sequence repeats (SSRs) were screened from the unigenes using RepeatMasker (v4.0.6) and MISA (v1.0) software tools, followed by the designing of SSRs flanking primers using BatchPrimer 3 (v1.0) software. RESULTS: The transcriptome produced a total of 69,822 unigenes, of which 46,175 annotated to the homologous proteins in the PANM-DB. The unigenes were also map...