Comparison of Helicobacter pylori positive and negative gastric cancer via multi-omics analysis

作者：Fumei Shang, Yinghao Cao, Lixin Wan, Zhonghai Ren, Xinghao Wang, Mudan Huang, Y. Guo · 发表于：mBio · 年份：2023 · DOI：10.1128/mbio.01531-23 · 被引用次数：13 · 研究领域：Helicobacter pylori-related gastroenterology studies、Gastric Cancer Management and Outcomes、Genetic factors in colorectal cancer

ABSTRACT Helicobacter pylori ( H. pylori ) has been regarded as a definite carcinogenic bacterium for gastric cancer (GC). This multi-omics research was designed to investigate the genetic, microbial, and metabolic changes of GC patients when they are infected with H. pylori . We first mined The Cancer Genome Atlas Stomach Adenocarcinoma (STAD) data to identify the key genes and critical pathways in H. pylori -positive individuals with GC compared to H. pylori -negative individuals with GC. Then, fresh stool samples were collected from GC individuals screened for eligibility, and we analyzed the microbial changes and metabolite alterations between H. pylori -positive and H. pylori -negative GC individuals. Finally, we tried to explore the interaction between key gut flora and metabolite changes in GC patients infected with H. pylori . We identified three genes (GCG, APOA1, and IGFBP1) with significant relevance to H. pylori infection, and the survival monogram based on the three H . pylori -related genes showed good predictive ability for overall survival among GC individuals. 16S rRNA sequencing showed that the abundance of Escherichia-Shigella, Bacteroides, Enterococcus , and Lactobacillus was upregulated in GC cases with H. pylori at the level of genus. There exists a great difference in alpha and beta diversity between H. pylori group and non- H . pylori group. The untargeted metabolome analysis identified 295 significant fecal metabolites, and the levels of penitrem E, a...