Avian influenza A viruses exhibit plasticity in sialylglycoconjugate receptor usage in human lung cells

作者：Chieh-Yu Liang, Iris Huang, Julianna Han, Boopathi Sownthirarajan, Katarina Kulhánková, Nathan B. Murray, Mehrnoush Taherzadeh Ghahfarrokhi, Stephanie Archer‐Hartmann, Lauren E. Pepi, Senthamizharasi Manivasagam, Jesse Plung, Miranda Sturtz, Yolanda Yu, O. A. Vogel, Matheswaran Kandasamy, Françoise A. Gourronc, Aloysius J. Klingelhutz, Biswa Choudhury, Lijun Rong, Jasmine T. Perez, Parastoo Azadi, Paul B. McCray, Sriram Neelamegham, Balaji Manicassamy · 发表于：Journal of Virology · 年份：2023 · DOI：10.1128/jvi.00906-23 · 被引用次数：7 · 研究领域：Influenza Virus Research Studies、interferon and immune responses、Immune Response and Inflammation

ABSTRACT Influenza A viruses (IAV) utilize sialic acid (Sia) containing cell surface glycoconjugates for host cell infection, and IAV strains from different host species show preferences for structurally distinct Sia at the termini of glycoconjugates. Various types of cell surface glycoconjugates (N-glycans, O-glycans, glycolipids) display significant diversity in both structure and carbohydrate composition. To define the types of sialylglycoconjugates that facilitate IAV infection, we utilized the CRISPR/Cas9 technique to truncate the three major types of glycoconjugates, either individually or in combination, by targeting glycosyltransferases essential to glycan biosynthesis in a human lung epithelial cell line. Our studies show that both human and avian IAV strains do not display strict preferences for a specific type of glycoconjugate. Interestingly, truncation of the three major types of glycoconjugates significantly decreased replication of human IAV strains, yet did not impact replication of avian IAV strains. Unlike human IAV strains, avian IAV strains were able to efficiently utilize other less prevalent shorter glycoconjugates such as sialyl Tn and sialyl T antigens. Taken together, our studies demonstrate that avian IAV strains utilize a broader repertoire of glycoconjugates for host cell infection as compared to human IAV strains. IMPORTANCE It is well known that influenza A viruses (IAV) initiate host cell infection by binding to sialic acid, a sugar molecule pre...