Comprehensive metabolome and transcriptome analyses demonstrate divergent anthocyanin and carotenoid accumulation in fruits of wild and cultivated loquats

作者：Wenbing Su, Changqing Zhu, Zhongqi Fan, Mingkun Huang, Lin Han, Xiuping Chen, Chaojun Deng, Yongping Chen, Yidan Kou, Zhihong Tong, Yaling Zhang, Changjie Xu, Shaoquan Zheng, Jimou Jiang · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1285456 · 被引用次数：16 · 研究领域：Plant biochemistry and biosynthesis、Plant Gene Expression Analysis、Horticultural and Viticultural Research

Eriobotrya is an evergreen fruit tree native to South-West China and adjacent countries. There are more than 26 loquat species known in this genus, while E. japonica is the only species yet domesticated to produce fresh fruits from late spring to early summer. Fruits of cultivated loquat are usually orange colored, in contrast to the red color of fruits of wild E. henryi (EH). However, the mechanisms of fruit pigment formation during loquat evolution are yet to be elucidated. To understand these, targeted carotenoid and anthocyanin metabolomics as well as transcriptomics analyses were carried out in this study. The results showed that β-carotene, violaxanthin palmitate and rubixanthin laurate, totally accounted for over 60% of the colored carotenoids, were the major carotenoids in peel of the orange colored ‘Jiefangzhong’ (JFZ) fruits. Total carotenoids content in JFZ is about 10 times to that of EH, and the expression levels of PSY , ZDS and ZEP in JFZ were 10.69 to 23.26 folds to that in EH at ripen stage. Cyanidin-3- O -galactoside and pelargonidin-3- O -galactoside were the predominant anthocyanins enriched in EH peel. On the contrary, both of them were almost undetectable in JFZ, and the transcript levels of F3H , F3’H , ANS , CHS and CHI in EH were 4.39 to 73.12 folds higher than that in JFZ during fruit pigmentation. In summary, abundant carotenoid deposition in JFZ peel is well correlated with the strong expression of PSY , ZDS and ZEP , while the accumulation of anth...