Gut microbiota-associated taurine metabolism dysregulation in a mouse model of Parkinson’s disease

作者：Can Cui, Huan Song, Yingying Han, Hongxiang Yu, Hongxia Li, Yumei Yang, Bei Zhang · 发表于：mSphere · 年份：2023 · DOI：10.1128/msphere.00431-23 · 被引用次数：21 · 研究领域：Gut microbiota and health、Parkinson's Disease Mechanisms and Treatments、Cannabis and Cannabinoid Research

ABSTRACT Parkinson’s disease (PD) is recognized as a multisystem disease concerning gastrointestinal (GI) dysfunction and microbiota dysbiosis. However, the mechanism by which microbial dysbiosis contributes to pathogenesis of PD and the relationship between gut microbes and metabolites remain to be deeply elucidated. This study aims to explore the altered microflora and serum metabolites in PD mice and their role in PD etiology. Herein, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP, 30 mg/kg) was injected intraperitoneally to establish the PD mice model. 16S rRNA sequencing and ultrahigh-performance liquid chromatography coupled with Q Exactive HF-X mass spectrometry were used to depict the profile of gut bacteria and metabolites. Phylogenetic investigation of communities by reconstruction of unobserved states 2 analysis was performed to elucidate the functional link between the microbe-metabolite axis and PD. We confirmed the MPTP-induced dopaminergic (DA) neuron loss accompanied by the GI dysfunction. Higher abundances of Aerococcus , Staphylococcus , and Ruminococcaceae , and lower abundances of Lactobacillus , Lachnospiraceae , and Adlercreutzia were identified in PD mice. Meanwhile, the differential metabolites concerning “taurine and hypotaurine metabolism” were markedly downregulated in PD mice. Furthermore, Lactobacillus , Adlercreutzia , and taurine-related metabolites showed the most significant correlation with pathological and GI performance of PD mice. The ...