Transcriptome and metabolome analyses of anthocyanin biosynthesis in post-harvest fruits of a full red-type kiwifruit (Actinidia arguta) ‘Jinhongguan’

作者：Lixia Ye, Fuxi Bai, Lei Zhang, Minmin Luo, Lei Gao, Zhi Wang, Jue Peng, Qinghong Chen, Xuan Luo · 发表于：Frontiers in Plant Science · 年份：2023 · DOI：10.3389/fpls.2023.1280970 · 被引用次数：21 · 研究领域：Plant Gene Expression Analysis、Phytochemicals and Antioxidant Activities、Plant biochemistry and biosynthesis

Anthocyanin is the main component of pigment in red-fleshed kiwifruit. ‘Jinhongguan’ is a new cultivar of Actinidia arguta with red peel and flesh after harvest. However, the specific types of anthocyanin in the ‘Jinhongguan’ fruit and its biosynthesis pathways remain largely unknown. Here, the total anthocyanin content in the fruit color conversion process was determined. The results showed that total anthocyanin content increased with the deepening color of the peel and flesh. To identify the genes related to anthocyanin biosynthesis and the types of anthocyanins in the ‘Jinhongguan’ fruit, a combined analysis of transcriptome and anthocyanin-targeted metabolome was carried out. A total of 5751 common differentially expressed genes (DEGs) at different stages of peel and flesh were identified, of which 2767 were common up-DEGs and 2976 were common down-DEGs. KEGG and GO enrichment analyses showed that the common up-DEGs were significantly enriched in anthocyanin synthesis-related pathways, suggesting some up-DEGs are involved in anthocyanin biosynthesis. In total, 29 metabolites were detected in the flesh by anthocyanin-targeted metabolome. Among these, nine were differential accumulation metabolites (DAMs) in comparison to red flesh vs green flesh. Six DAMs were up-regulated, with five of them were cyanidins. The content of cyanidin-3-O-galactoside was much higher than that of other DAMs, making it the main pigment in ‘Jinhongguan’. Moreover, a total of 36 anthocyanin synth...