Metabolic Engineering of Pichia pastoris for High-Level Production of Lycopene

作者：Xinying Zhang, Shuting Chen, Ying Lin, Wenjie Li, Denggang Wang, Shupeng Ruan, Yuxin Yang, Shuli Liang · 发表于：ACS Synthetic Biology · 年份：2023 · DOI：10.1021/acssynbio.3c00294 · 被引用次数：64 · 研究领域：CRISPR and Genetic Engineering、Microbial Metabolic Engineering and Bioproduction、Plant biochemistry and biosynthesis

Lycopene is widely used in cosmetics, food, and nutritional supplements. Microbial production of lycopene has been intensively studied. However, few metabolic engineering studies on Pichia pastoris have been aimed at achieving high-yield lycopene production. In this study, the CRISPR/Cpf1-based gene repression system was developed and the gene editing system was optimized, which were applied to improve lycopene production successfully. In addition, the sterol regulatory element-binding protein SREBP (Sre) was used for the regulation of lipid metabolic pathways to promote lycopene overproduction in P. pastoris for the first time. The final engineered strain produced lycopene at 7.24 g/L and 75.48 mg/g DCW in fed-batch fermentation, representing the highest lycopene yield in P. pastoris reported to date. These findings provide effective strategies for extended metabolic engineering assisted by the CRISPR/Cpf1 system and new insights into metabolic engineering through transcriptional regulation of related metabolic pathways to enhance carotenoid production in P. pastoris .