Microbial controls over soil priming effects under chronic nitrogen and phosphorus additions in subtropical forests

作者：Jian Li, Zhanfeng Liu, Mingkang Jin, Wei Zhang, Hans Lambers, Dafeng Hui, Chao Liang, Jing Zhang, Donghai Wu, Jordi Sardans, Josep Peñuelas, Daniel F. Petticord, David W. Frey, Yong‐Guan Zhu · 发表于：The ISME Journal · 年份：2023 · DOI：10.1038/s41396-023-01523-9 · 被引用次数：61 · 研究领域：Soil Carbon and Nitrogen Dynamics、Forest Ecology and Biodiversity Studies、Ecology and Vegetation Dynamics Studies

Abstract The soil priming effect (PE), defined as the modification of soil organic matter decomposition by labile carbon (C) inputs, is known to influence C storage in terrestrial ecosystems. However, how chronic nutrient addition, particularly in leguminous and non-leguminous forests, will affect PE through interaction with nutrient (e.g., nitrogen and phosphorus) availability is still unclear. Therefore, we collected soils from leguminous and non-leguminous subtropical plantations across a suite of historical nutrient addition regimes. We added 13C-labeled glucose to investigate how background soil nutrient conditions and microbial communities affect priming and its potential microbial mechanisms. Glucose addition increased soil organic matter decomposition and prompted positive priming in all soils, regardless of dominant overstory tree species or fertilizer treatment. In non-leguminous soil, only combined nitrogen and phosphorus addition led to a higher positive priming than the control. Conversely, soils beneath N-fixing leguminous plants responded positively to P addition alone, as well as to joint NP addition compared to control. Using DNA stable-isotope probing, high-throughput quantitative PCR, enzyme assays and microbial C substrate utilization, we found that positive PE was associated with increased microbial C utilization, accompanied by an increase in microbial community activity, nutrient-related gene abundance, and enzyme activities. Our findings suggest that t...