Geraniol Suppresses Oxidative Stress, Inflammation, and Interstitial Collagenase to Protect against Inflammatory Arthritis

作者：Muhammad Nasir Hayat Malik, Muhammad Tahir, Tariq G. Alsahli, Md. Mahedi Hassan Tusher, Sami I. Alzarea, Bader Alsuwayt, Shah Jahan, Hesham A. M. Gomaa, Mohamed E. Shaker, Muhammad Ali, İrfan Anjum, Muhammad Tariq Khan, Muhammad Roman, Ramla Shabbir · 发表于：ACS Omega · 年份：2023 · DOI：10.1021/acsomega.3c04684 · 被引用次数：22 · 研究领域：Rheumatoid Arthritis Research and Therapies、Natural product bioactivities and synthesis、Natural Compounds in Disease Treatment

models. This study was therefore designed to evaluate the antiarthritic potential of GER in complete Freund's adjuvant (CFA)-induced inflammatory arthritis (IA) model in rats. IA was induced by intraplantar injection of CFA (0.1 mL), and a week after CFA administration, rats were treated with various doses of methotrexate (MTX; 1 mg/kg) or GER (25, 50, and 100 mg/kg). Treatments were given on every alternate day, and animals were sacrificed on the 35th day. Paw volume, histopathological, hematological, radiographic, and qPCR analyses were performed to analyze the severity of the disease. GER significantly reduced paw edema after 35 days of treatment, and these results were comparable to the MTX-treated group. GER-treated animals displayed a perfect joint structure with minimal inflammation and no signs of cartilage or bone damage. Moreover, GER restored red blood cell and hemoglobin levels, normalized erythrocyte sedimentation rate, platelet, and c-reactive protein values, and also attenuated the levels of rheumatoid factor. RT-qPCR analysis demonstrated that GER decreased mRNA expression of pro-inflammatory cytokines like tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta. GER also down-regulated the transcript levels of cyclooxygenase-2 (COX-2), microsomal prostaglandin E synthase-1, prostaglandin D2 synthase, and interstitial collagenase (MMP-1). Molecular docking of GER with COX-2, TNF-α, and MMP-1 also revealed that the antiarthritic effects of GER could be due t...