Dynamics of oxidative stress and immune responses in neonatal calves during diarrhea

作者：Zilin Fu, Yi Yang, Lu Ma, Nilusha Malmuthuge, Le Luo Guan, Dengpan Bu · 发表于：Journal of Dairy Science · 年份：2023 · DOI：10.3168/jds.2023-23630 · 被引用次数：45 · 研究领域：Animal health and immunology、Viral gastroenteritis research and epidemiology、Effects of Environmental Stressors on Livestock

Oxidative stress is the imbalanced redox status between oxidants production and their scavengers leading to intestinal physiological dysfunction. However, the role of systemic and local oxidative status during neonatal calf diarrhea is not known. This study assessed systemic (serum) and local (fecal) oxidative status when calves either naturally developed diarrhea or naturally recovered. Healthy calves were enrolled in the study at age of d 18 and their health status were monitored from the enrollment. Based on their enteric health status on D21 and D28, calves were grouped as continuous diarrhea from D21 to D28 (DD group, n=14), diarrhea at D21 but recovered at D28 (DH group, n=19), healthy at D21 but developed diarrhea at D28 (HD group, n=15), and healthy throughout the study (HH group, n=16). Serum and fecal samples were collected at D21 and D28 from all calves in the morning 2 h after feeding. Dynamics of oxidative stress indicators including reactive oxygen species (ROS), malondialdehyde (MDA), H 2 O 2 , 8-hydroxylated deoxyguanosine (8-OHDG), glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (CAT), and total antioxidant capacity (T-AOC) and inflammatory indicators TNF-α, IL-1β, IL-4, IL-6, IL-10 and IFN-γ were evaluated using serum samples. In addition, fecal oxidative stress indicators ROS and MDA were measured. Serum ROS, MDA, 8-OHDG, as well as fecal ROS and MDA were higher, while serum CAT and H 2 O 2 were lower in diarrheic calves than those of hea...