IgM antibody responses against Plasmodium antigens in neotropical primates in the Brazilian Atlantic Forest

作者：Gabriela Maíra Pereira de Assis, Denise Anete Madureira de Alvarenga, Luisa Braga e Souza, Juan Camilo Sánchez‐Arcila, Eduardo Fernandes e Silva, Anielle de Pina-Costa, Gustavo Henrique Pereira Gonçalves, Júlio César de Junior Souza, Ana Júlia Dutra Nunes, Alcides Pissinatti, Sílvia Bahadian Moreira, Letícia de Menezes Torres, Helena L. Costa, Herlandes da Penha Tinoco, Valéria do Socorro Pereira, Irene S. Soares, Taís Nóbrega de Sousa, Francis B. Ntumngia, John H. Adams, Flora Satiko Kano, Zelinda Maria Braga Hirano, Lilian Rose Pratt-Riccio, Cláudio Tadeu Daniel‐Ribeiro, Joseli Oliveira Ferreira, Luzia H. Carvalho, Cristiana Ferreira Alves de Brito · 发表于：Frontiers in Cellular and Infection Microbiology · 年份：2023 · DOI：10.3389/fcimb.2023.1169552 · 被引用次数：3 · 研究领域：Malaria Research and Control、Bird parasitology and diseases、Primate Behavior and Ecology

Introduction: Zoonotic transmission is a challenge for the control and elimination of malaria. It has been recorded in the Atlantic Forest, outside the Amazon which is the endemic region in Brazil. However, only very few studies have assessed the antibody response, especially of IgM antibodies, in Neotropical primates (NP). Therefore, in order to contribute to a better understanding of the immune response in different hosts and facilitate the identification of potential reservoirs, in this study, naturally acquired IgM antibody responses against Plasmodium antigens were evaluated, for the first time, in NP from the Atlantic Forest. Methods: species and different erythrocytic stage antigens were detected by ELISA. Results: vivax erythrocytic stage antigen. Levels of antibodies against PvAMA-1 were the highest compared to the other antigens. All families of NP showed IgM antibodies against CSP peptides, and, most strikingly, against erythrocytic stage antigens. Generalized linear models demonstrated that IgM positivity against PvCSP and PvAMA-1 was associated with PCR-detectable blood-stage malaria infection and the host being free-living. Interestingly, animals with IgM against both PvCSP and PvAMA-1 were 4.7 times more likely to be PCR positive than animals that did not have IgM for these two antigens simultaneously. Discussion: spp. antigens are present in NP from the Atlantic Forest. High seroprevalence and antibody levels against blood-stage antigens were observed, which h...