The two-component system CpxAR is required for the high potassium stress survival of Actinobacillus pleuropneumoniae

作者：Jiajia Wan, Rui Zhang, Yizhen Jia, Tingting Xie, Lu Dai, Qing Yao, Wendie Zhang, Huasong Xiao, Xuejun Gao, Jing Huang, Weicheng Bei, Feng Liu · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1259935 · 被引用次数：7 · 研究领域：Microbial infections and disease research、Bacteriophages and microbial interactions、Aquaculture disease management and microbiota

Introduction Actinobacillus pleuropneumoniae is an important respiratory pathogen, which can cause porcine contagious pleuropneumonia and lead to great economic losses to worldwide swine industry. High potassium is an adverse environment for bacteria, which is not conducive to providing turgor pressure for cell growth and division. Two-component system CpxAR is an important regulatory system of bacteria in response to environmental changes, which is involved in a variety of biological activities, such as antibiotic resistance, periplasmic protein folding, peptidoglycan metabolism and so on. Methods However, little is known about the role of CpxAR in high potassium stress in A. pleuropneumoniae . Here, we showed that CpxAR is critical for cell division of A. pleuropneumoniae under high potassium (K + ) stress. Results qRT-PCR analysis found that CpxAR positively regulated the cell division genes ftsEX. In addition, we also demonstrated that CpxR-P could directly bind the promoter region of the cell division gene ftsE by EMSA. Discussion In conclusion, our results described a mechanism where CpxAR adjusts A. pleuropneumoniae survival under high-K + stress by upregulating the expression of the cell division proteins FtsE and FtsX. These findings are the first to directly demonstrate CpxAR-mediated high-K + tolerance, and to investigate the detailed molecular mechanism.