Knockdown of LMNA inhibits Akt/β-catenin-mediated cell invasion and migration in clear cell renal cell carcinoma cells

作者：Hui Xin, Yu Tang, Yanhong Jin, Hu‐Li Li, Tian Yu, Yu Cong, Zeju Zhao, Mingsong Wu, You Fu Pan · 发表于：Cell Adhesion & Migration · 年份：2023 · DOI：10.1080/19336918.2023.2260644 · 被引用次数：5 · 研究领域：Nuclear Structure and Function、RNA Research and Splicing、Renal and related cancers

The LMNA gene encoding lamin A/C is amplified in some clear cell renal cell carcinoma (ccRCC) samples. Our data showed that depletion of the tumor suppressor PBRM1 can upregulate lamin A/C levels, and lamin A/C could interact with PBRM1. However, the role of lamin A/C in ccRCC is not yet fully understood. Our functional assays showed that although the proliferation ability was slightly impaired after LMNA depletion, the migration and invasion of ccRCC cells were significantly inhibited. This suppression was accompanied by a reduction in MMP2, MMP9, AKT/p-AKT, and Wnt/β-catenin protein levels. Our data therefore suggest that lamin A/C, as an interaction partner of the tumor suppressor PBRM1, plays a crucial role in tumor invasion and metastasis in ccRCC.