The anti-hepatocellular carcinoma effect of Aidi injection was related to the synergistic action of cantharidin, formononetin, and isofraxidin through BIRC5, FEN1, and EGFR

作者：Shan Lu, Jiaqi Huang, Jingyuan Zhang, Chao Wu, Zhihong Huang, Xiaoyu Tao, Leiming You, Antony Stalin, Meilin Chen, Jiaqi Li, Yingying Tan, Zhishan Wu, Libo Geng, Zhiqi Li, Qiqi Fan, Pengyun Liu, Yifan Lin, Chongjun Zhao, Jiarui Wu · 发表于：Journal of Ethnopharmacology · 年份：2023 · DOI：10.1016/j.jep.2023.117209 · 被引用次数：17 · 研究领域：Beetle Biology and Toxicology Studies、Natural Compounds in Disease Treatment、Histone Deacetylase Inhibitors Research

ETHNOPHARMACOLOGICAL RELEVANCE: Aidi injection (ADI) is a popular anti-tumor Chinese patent medicine, widely used in clinics for the treatment of hepatocellular carcinoma (HCC) with remarkable therapeutic effects through multiple targets and pathways. However, the scientific evidence of the synergistic role of the complex chemical component system and the potential mechanism for treating diseases are ignored and remain to be elucidated. AIM OF THE STUDY: This study aimed to elucidate and verify the cooperative association between the potential active ingredient of ADI, which is of significance to enlarge our understanding of its anti-HCC molecular mechanisms. MATERIALS AND METHODS: Firstly, the anti-HCC effect of ADI was evaluated in various HCC cells and the zebrafish xenograft model. Subsequently, a variety of bioinformatic technologies, including network pharmacology, weighted gene co-expression network analysis (WGCNA), meta-analysis of gene expression profiles, and pathway enrichment analysis were performed to construct the competitive endogenous RNA (ceRNA) network of ADI intervention in HCC and to establish the relationship between the critical targets/pathways and the key corresponding components, which were involved in ADI against HCC in a synergistic way and were validated by molecular biology experiments. RESULTS: ADI exerted remarkable anti-HCC in vitro cells and in vivo zebrafish model, especially that the Hep 3B2.1-7 cell showed substantial sensibility to ADI. T...