Prediction of cell-cell communication patterns of dorsal root ganglion cells: single-cell RNA sequencing data analysis

作者：Yan‐Na Lian, Cheng Wu, Li Liu, Xiang‐Yao Li · 发表于：Neural Regeneration Research · 年份：2023 · DOI：10.4103/1673-5374.384067 · 被引用次数：11 · 研究领域：Dermatology and Skin Diseases、Exercise and Physiological Responses、Stress Responses and Cortisol

Abstract JOURNAL/nrgr/04.03/01300535-202406000-00042/inline-graphic1/v/2025-09-25T172020Z/r/image-tiff Dorsal root ganglion neurons transmit peripheral somatic information to the central nervous system, and dorsal root ganglion neuron excitability affects pain perception. Dorsal root ganglion stimulation is a new approach for managing pain sensation. Knowledge of the cell-cell communication among dorsal root ganglion cells may help in the development of new pain and itch management strategies. Here, we used the single-cell RNA-sequencing (scRNA-seq) database to investigate intercellular communication networks among dorsal root ganglion cells. We collected scRNA-seq data from six samples from three studies, yielding data on a total of 17,766 cells. Based on genetic profiles, we identified satellite glial cells, Schwann cells, neurons, vascular endothelial cells, immune cells, fibroblasts, and vascular smooth muscle cells. Further analysis revealed that eight types of dorsal root ganglion neurons mediated proprioceptive, itch, touch, mechanical, heat, and cold sensations. Moreover, we predicted several distinct forms of intercellular communication among dorsal root ganglion cells, including cell-cell contact, secreted signals, extracellular matrix, and neurotransmitter-mediated signals. The data mining predicted that Mrgpra3 -positive neurons robustly express the genes encoding the adenosine Adora2b (A2B) receptor and glial cell line-derived neurotrophic factor family receptor ...