Minimal residual disease assessment in B ‐cell precursor acute lymphoblastic leukemia by semi‐automated identification of normal hematopoietic cells: A EuroFlow study

作者：Martijn W. C. Verbeek, Beatriz Rodríguez‐Roca, Łukasz Sędek, Anna Laqua, Chiara Buracchi, Malicorne Buysse, Michaela Reiterová, Elen Oliveira, Daniela Morf, Sjoerd R. Oude Alink, Susana Bárrena, Saskia Kohlscheen, Stefan Nierkens, Mattias Hofmans, Paula Fernández, Elaine Sobral de Costa, Ester Mejstříková, Tomasz Szczepański, Łukasz Słota, Monika Brüggemann, Giuseppe Gaipa, Georgiana Grigore, Jacques J. M. van Dongen, Alberto Órfão, Vincent H. J. van der Velden · 发表于：Cytometry Part B Clinical Cytometry · 年份：2023 · DOI：10.1002/cyto.b.22143 · 被引用次数：10 · 研究领域：Acute Lymphoblastic Leukemia research、Cancer Genomics and Diagnostics、Hematopoietic Stem Cell Transplantation

Presence of minimal residual disease (MRD), detected by flow cytometry, is an important prognostic biomarker in the management of B-cell precursor acute lymphoblastic leukemia (BCP-ALL). However, data-analysis remains mainly expert-dependent. In this study, we designed and validated an Automated Gating & Identification (AGI) tool for MRD analysis in BCP-ALL patients using the two tubes of the EuroFlow 8-color MRD panel. The accuracy, repeatability, and reproducibility of the AGI tool was validated in a multicenter study using bone marrow follow-up samples from 174 BCP-ALL patients, stained with the EuroFlow BCP-ALL MRD panel. In these patients, MRD was assessed both by manual analysis and by AGI tool supported analysis. Comparison of MRD levels obtained between both approaches showed a concordance rate of 83%, with comparable concordances between MRD tubes (tube 1, 2 or both), treatment received (chemotherapy versus targeted therapy) and flow cytometers (FACSCanto versus FACSLyric). After review of discordant cases by additional experts, the concordance increased to 97%. Furthermore, the AGI tool showed excellent intra-expert concordance (100%) and good inter-expert concordance (90%). In addition to MRD levels, also percentages of normal cell populations showed excellent concordance between manual and AGI tool analysis. We conclude that the AGI tool may facilitate MRD analysis using the EuroFlow BCP-ALL MRD protocol and will contribute to a more standardized and objective MRD...