Effects of Lactiplantibacillus plantarum on oxidative stress, mitophagy, and NLRP3 inflammasome activation in broiler breast meat

作者：Junmeng Yuan, Fan Zhao, Yu Liu, Huawei Liu, Kai Zhang, Xinyu Tian, Yuxin Mu, Jinshan Zhao, Yang Wang · 发表于：Poultry Science · 年份：2023 · DOI：10.1016/j.psj.2023.103128 · 被引用次数：20 · 研究领域：Autophagy in Disease and Therapy、Inflammasome and immune disorders、Toxoplasma gondii Research Studies

Poultry meat has a high polyunsaturated fatty acids content, making it vulnerable to oxidative stress. Mitophagy participates in the regulation of oxidative stress and the nucleotide-binding and oligomerization domain (NOD)-like receptor family as well as pyrin domain-containing protein 3 (NLRP3) inflammasome activation. Lactiplantibacillus plantarum P8 (P8) is a probiotic strain with an antioxidant capacity. In the present study, we investigated the effects of P8 on oxidative stress, mitochondrial function, mitophagy, and NLRP3 inflammasome in the breast meat of oxidatively stressed broilers. Four hundred one-day-old male broilers were assigned to a 2 × 2 factorial design with two P8 levels (0 or 1 × 108 cfu/g), either with or without dexamethasone (DEX) injection, for a 21-day experimental period. DEX was injected intraperitoneally once daily from days 16 to 21. The breast meat was collected on day 21. The results showed that P8 supplementation decreased malondialdehyde (MDA) levels, increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and activated the Keap1-Nrf2 pathway in DEX-injected broilers. Moreover, P8 supplementation down-regulated mitochondrial DNA (mtDNA) copy number and increased the expressions of peroxisome proliferator-activated receptor gamma coactivator 1α (PGC-1α), silent information regulator 1 (SIRT1), mitochondrial fusion protein 1 (Mfn1) and optic atrophy protein 1 (OPA1) in DEX-treated broilers. In addition, the decreased mitophag...