Control of arbuscule development by a transcriptional negative feedback loop in Medicago

作者：Qiang Zhang, Shuangshuang Wang, Qiujin Xie, Yuanjun Xia, Lei Lü, Mingxing Wang, Gang Wang, Siyu Long, Yunfei Cai, Ling Xu, Ertao Wang, Yina Jiang · 发表于：Nature Communications · 年份：2023 · DOI：10.1038/s41467-023-41493-2 · 被引用次数：42 · 研究领域：Mycorrhizal Fungi and Plant Interactions、Plant nutrient uptake and metabolism、Legume Nitrogen Fixing Symbiosis

Most terrestrial plants establish a symbiosis with arbuscular mycorrhizal fungi (AMF), which provide them with lipids and sugars in exchange for phosphorus and nitrogen. Nutrient exchange must be dynamically controlled to maintain a mutually beneficial relationship between the two symbiotic partners. The WRI5a and its homologues play a conserved role in lipid supply to AMF. Here, we demonstrate that the AP2/ERF transcription factor MtERM1 binds directly to AW-box and AW-box-like cis-elements in the promoters of MtSTR2 and MtSTR, which are required for host lipid efflux and arbuscule development. The EAR domain-containing transcription factor MtERF12 is also directly activated by MtERM1/MtWRI5a to negatively regulate arbuscule development, and the TOPLESS co-repressor is further recruited by MtERF12 through EAR motif to oppose MtERM1/MtWRI5a function, thereby suppressing arbuscule development. We therefore reveal an ERM1/WRI5a-ERF12-TOPLESS negative feedback loop that enables plants to flexibly control nutrient exchange and ensure a mutually beneficial symbiosis.