Turning a Sesquiterpene Synthase into a Di- and Sesterterpene Synthase

作者：Houchao Xu, Jeroen S. Dickschat · 发表于：ACS Catalysis · 年份：2023 · DOI：10.1021/acscatal.3c03740 · 被引用次数：20 · 研究领域：Plant biochemistry and biosynthesis、Natural product bioactivities and synthesis、Microbial Natural Products and Biosynthesis

The sesquiterpene synthase 7- epi -α-eudesmol synthase is widespread in streptomycetes. Several active site residues that were identified by an alignment of their amino acid sequence to the sequence of the structurally known selina-4(15),7(11)-diene synthase were targeted by site-directed mutagenesis. In these experiments, the active site cavity was widened through exchange of large with small residues, resulting in the discovery of two key residues for which such exchanges turned 7- epi -α-eudesmol synthase into a di- or sesterterpene synthase.