From the perspective of rumen microbiome and host metabolome, revealing the effects of feeding strategies on Jersey Cows on the Tibetan Plateau

作者：Niuniu Yuan, Yicui Wang, Qihao Pan, Zhao Li, Qi Xiao, Shihao Sun, Suolang Quji, Luobu Ciren, Danzeng Luosang, Yanxin Liu, Liyang Zhang, Tengyun Gao, Zhuza Basang, Hongxia Lian, Yu Sun · 发表于：PeerJ · 年份：2023 · DOI：10.7717/peerj.16010 · 被引用次数：4 · 研究领域：Ruminant Nutrition and Digestive Physiology、Gout, Hyperuricemia, Uric Acid、Rangeland Management and Livestock Ecology

Background Previous studies have discussed the effects of grazing and house feeding on yaks during the cold season when forage is in short supply, but there is limited information on the effects of these feeding strategies on Jersey cows introduced to the Tibetan Plateau. The objective of this study was to use genomics and metabolomics analyses to examine changes in rumen microbiology and organism metabolism of Jersey cows with different feeding strategies. Methods We selected 12 Jersey cows with similar body conditions and kept them for 60 days under grazing ( n = 6) and house-feeding ( n = 6) conditions. At the end of the experiment, samples of rumen fluid and serum were collected from Jersey cows that had been fed using different feeding strategies. The samples were analyzed for rumen fermentation parameters, rumen bacterial communities, serum antioxidant and immunological indices, and serum metabolomics. The results of the study were examined to find appropriate feeding strategies for Jersey cows during the cold season on the Tibetan plateau. Results The results of rumen fermentation parameters showed that concentrations of acetic acid, propionic acid, and ammonia nitrogen in the house-feeding group (Group B) were significantly higher than in the grazing group (Group G) ( P < 0.05). In terms of the rumen bacterial community 16S rRNA gene, the Chao1 index was significantly higher in Group G than in Group B ( P = 0.038), while observed species, Shannon and Simpson indice...