A screening identifies harmine as a novel antibacterial compound against Ralstonia solanacearum

作者：Hongkai Xia, Yanxia Huang, Ruoyu Wu, Xin Tang, Jun Cai, Shunxiang Li, Lin Jiang, Dousheng Wu · 发表于：Frontiers in Microbiology · 年份：2023 · DOI：10.3389/fmicb.2023.1269567 · 被引用次数：18 · 研究领域：Plant Pathogenic Bacteria Studies、Phytochemistry and biological activities of Ficus species、Plant-Microbe Interactions and Immunity

Ralstonia solanacearum , the causal agent of bacterial wilt, is a devastating plant pathogenic bacterium that infects more than 450 plant species. Until now, there has been no efficient control strategy against bacterial wilt. In this study, we screened a library of 100 plant-derived compounds for their antibacterial activity against R. solanacearum . Twelve compounds, including harmine, harmine hydrochloride, citral, vanillin, and vincamine, suppressed bacterial growth of R. solanacearum in liquid medium with an inhibition rate higher than 50%. Further focus on harmine revealed that the minimum inhibitory concentration of this compound is 120 mg/L. Treatment with 120 mg/L of harmine for 1 and 2 h killed more than 90% of bacteria. Harmine treatment suppressed the expression of the virulence-associated gene xpsR . Harmine also significantly inhibited biofilm formation by R. solanacearum at concentrations ranging from 20 mg/L to 60 mg/L. Furthermore, application of harmine effectively reduced bacterial wilt disease development in both tobacco and tomato plants. Collectively, our results demonstrate the great potential of plant-derived compounds as antibacterial agents against R. solanacearum , providing alternative ways for the efficient control of bacterial wilt.