Wheat gibberellin oxidase genes and their functions in regulating tillering

作者：Ting Wang, Junchang Li, Yumei Jiang, Jıng Zhang, Yongjing Ni, Peipei Zhang, Ziping Yao, Zhixin Jiao, Huijuan Li, Lei Li, Yufan Niu, Qiao‐Yun Li, Guihong Yin, Jishan Niu · 发表于：PeerJ · 年份：2023 · DOI：10.7717/peerj.15924 · 被引用次数：12 · 研究领域：Plant Molecular Biology Research、Plant nutrient uptake and metabolism、Plant responses to water stress

Multiple genetic factors control tillering, a key agronomy trait for wheat ( Triticum aestivum L.) yield. Previously, we reported a dwarf-monoculm mutant ( dmc ) derived from wheat cultivar Guomai 301, and found that the contents of gibberellic acid 3 (GA 3 ) in the tiller primordia of dmc were significantly higher. Transcriptome analysis indicated that some wheat gibberellin oxidase ( TaGAox ) genes TaGA20ox-A2 , TaGA20ox-B2 , TaGA3ox-A2 , TaGA20ox-A4 , TaGA2ox-A10 and TaGA2ox-B10 were differentially expressed in dmc . Therefore, this study systematically analyzed the roles of gibberellin oxidase genes during wheat tillering. A total of 63 TaGAox genes were identified by whole genome analysis. The TaGAoxs were clustered to four subfamilies, GA20oxs, GA2oxs, GA3oxs and GA7oxs, including seven subgroups based on their protein structures. The promoter regions of TaGAox genes contain a large number of cis -acting elements closely related to hormone, plant growth and development, light, and abiotic stress responses. Segmental duplication events played a major role in TaGAoxs expansion. Compared to Arabidopsis , the gene collinearity degrees of the GAoxs were significantly higher among wheat, rice and maize. TaGAox genes showed tissue-specific expression patterns. The expressions of TaGAox genes ( TaGA20ox-B2 , TaGA7ox-A1 , TaGA2ox10 and TaGA3ox-A2 ) were significantly affected by exogenous GA 3 applications, which also significantly promoted tillering of Guomai 301, but didn’t pr...