Mechanisms and biotechnological applications of transcription factors

作者：Hehe He, Yang Mingfei, Siyu Li, Gaoyang Zhang, Zhongyang Ding, Liang Zhang, Guiyang Shi, Youran Li · 发表于：Synthetic and Systems Biotechnology · 年份：2023 · DOI：10.1016/j.synbio.2023.08.006 · 被引用次数：68 · 研究领域：Genomics and Chromatin Dynamics、Viral Infectious Diseases and Gene Expression in Insects、CRISPR and Genetic Engineering

Transcription factors play an indispensable role in maintaining cellular viability and finely regulating complex internal metabolic networks. These crucial bioactive functions rely on their ability to respond to effectors and concurrently interact with binding sites. Recent advancements have brought innovative insights into the understanding of transcription factors. In this review, we comprehensively summarize the mechanisms by which transcription factors carry out their functions, along with calculation and experimental-based methods employed in their identification. Additionally, we highlight recent achievements in the application of transcription factors in various biotechnological fields, including cell engineering, human health, and biomanufacturing. Finally, the current limitations of research and provide prospects for future investigations are discussed. This review will provide enlightening theoretical guidance for transcription factors engineering.