Fluorescence “light-up” sensor based on ligand/SiO2@NH2@cyanuric chloride nanoparticle interactions in alliance with salt dehydration for berberine detection

作者：Liang Qi, Ying Gao, Shuya Pei, Pin Gong, Xiangna Chang, Hui Yan, Xinting Zhang · 发表于：Spectrochimica Acta Part A Molecular and Biomolecular Spectroscopy · 年份：2023 · DOI：10.1016/j.saa.2023.123330 · 被引用次数：9 · 研究领域：Berberine and alkaloids research、Antibiotics Pharmacokinetics and Efficacy、Drug Transport and Resistance Mechanisms

Berberine (BBR) is an important anti-inflammatory drug for the treatment of intestinal diseases. The quantification of BBR is required in clinical medicine because long-term or excessive intake can lead to drug resistance and adverse effects. In this study, SiO 2 @NH 2 @cyanuric chloride (CNCl) nanoparticles (NPs) were successfully prepared by covalently incorporating CNCl onto the surface of SiO 2 NPs. Furthermore, a novel fluorescence “light-up” sensor for assaying BBR was established based on the interaction between BBR and SiO 2 @NH 2 @CNCl NPs. Although BBR was non-emissive in aqueous media, its fluorescence was considerably augmented because of the interaction with the as-prepared SiO 2 @NH 2 @CNCl NPs, and the enhancement factor was approximately three times larger than that of pure SiO 2 NPs. Compared with SiO 2 NPs, SiO 2 @NH 2 @CNCl NPs can interact with BBR through electrostatic interactions and π-π stacking. These interactions restricted the intramolecular motion and charge transfer of BBR, resulting in fluorescence enhancement. The sensor was sensitive, with a linear response over a concentration range of 25–2500 nM ( R 2 = 0.9905) and a detection limit (3 σ / k ) of 4.7 nM, and it had good selectivity for BBR in the presence of bovine serum albumin, amino acids, and metal ions. When the sensor was applied to real serum samples, rapid extraction and salt dehydration occurred to improve the efficiency of pretreatment, and satisfactory standard recovery rates (95%–...