Study on Optimal Extraction and Hypoglycemic Effect of Quercetin

作者：Lei Zhang, Mingyue Pan, Tao Li, Zhi‐Min Jin, Zhu Liu, Qiu-yue Liu, Yong Liu, Jiayuan Ding, Huan Jiang, Xingchen Hou · 发表于：Evidence-based Complementary and Alternative Medicine · 年份：2023 · DOI：10.1155/2023/8886503 · 被引用次数：7 · 研究领域：Tea Polyphenols and Effects、Natural Antidiabetic Agents Studies、Biochemical effects in animals

Quercetin was extracted from Portulaca oleracea L. through biphasic acid hydrolysis to investigate its potential as a suppressor of dipeptidyl peptidase IV (DPP‐IV) and its hypoglycemic effect in type 2 diabetic mice. The extraction procedure was optimized utilizing the response surface method (RSM) in a single‐factor experimental setting. An extraction efficiency of 0.675% was achieved using the following optimized parameters: 0.064 mol/L vitriol, 1 : 109.155 solid‐liquid ratio, and 21.408 min ultrasonication. Overall, findings indicate the effectiveness of quercetin extraction. A mouse model for type 2 diabetes was established to receive oral treatment with various quercetin concentrations for 8 weeks. Fasting blood glucose (FBG) and the DPP‐IV activity in the serum were significantly reduced. The weight and insulin levels of the mice in the quercetin group were raised compared to those in the model group ( P < 0.01). Quercetin dose‐dependently inhibited postprandial blood glucose excursions, as demonstrated by the oral glucose tolerance test. These results confirmed that quercetin has hypoglycemic effects and considerably improves insulin sensitivity via DPP‐IV targeting.